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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Measurement of the distribution of an equivalent of cell dry mass using quantitative phase contrast of the CCHM
 Kovářová, Klára ; Čolláková, Jana (referee) ; Křížová, Aneta (advisor)
Subject of this bachelor's thesis is to create an overview of conversions of phase profile to the values of dry mass and to realize experiments verifying the importance of dry mass. In the theoretical part, the history of the holographic microscopy and the development of the holographic microscopy on IPE FME BUT in Brno are summarized. The transmission holographic microscope is also described. This thesis covers its construction description and basic working instructions. The theoretical part also deals with the importance of image phase. Two approaches are presented for the interpretation of the phase delay and there is research on phase conversions to dry cell mass (according to H. G. Davies and M. H. F. Wilkins, G. Popescue, G. Dunn and D. Zicha). In the experimental part cultivation of cells, preparation of observation sample and processing of data are described. This part of thesis is also focused on laboratory experiments. Cells K2 (full name LW13K2) were observed in all experiments. First experiments deal with nutritional deprivation of cells and later with cell growth.
Detailed record
Measurement of the distribution of an equivalent of cell dry mass using quantitative phase contrast of the CCHM
 Kovářová, Klára ; Čolláková, Jana (referee) ; Křížová, Aneta (advisor)
Subject of this bachelor's thesis is to create an overview of conversions of phase profile to the values of dry mass and to realize experiments verifying the importance of dry mass. In the theoretical part, the history of the holographic microscopy and the development of the holographic microscopy on IPE FME BUT in Brno are summarized. The transmission holographic microscope is also described. This thesis covers its construction description and basic working instructions. The theoretical part also deals with the importance of image phase. Two approaches are presented for the interpretation of the phase delay and there is research on phase conversions to dry cell mass (according to H. G. Davies and M. H. F. Wilkins, G. Popescue, G. Dunn and D. Zicha). In the experimental part cultivation of cells, preparation of observation sample and processing of data are described. This part of thesis is also focused on laboratory experiments. Cells K2 (full name LW13K2) were observed in all experiments. First experiments deal with nutritional deprivation of cells and later with cell growth.
Detailed record

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